7.1.3.1 Construction of AAV

Adeno-associated vectors are prepared by replacing the capsid genes with the genes of interest. The vector consists of recombinant AAV plasmid DNA and AAV helper plasmid that encodes the AA capsid protein.  It also requires either wild type adenovirus or HSV and cell line for viral propagation.  Cells are first infected with the wild type adenovirus or HSV, and they are then recombined. Both the recombinant AAV vector plasmid DNA and the non-rescuable AAV helper plasmid are transfected into the cell. The cells produce mature recombinant AAV vectors and wild type adenovirus or HSV. The wild type adenovirus or HSV is then removed.