7.1.2.6 Advantages and Disadvantages of Adenovirus

Its advantages of the adenoviral vectors are their efficiency in transduction and capable of inserting DNA of size up to 8kb, and infects both replicating and differentiated cells. Since it is not integrative with the host chromosome, therefore, it causes no mutagenic effect caused by the random integration into the host genome. The disadvantages include that the expression is transient since it is not integrative so that the therapy would need to be repeated.  Adenovirus is a common pathogen to human and in vivo delivery it provokes a strong immune response.  Recently adenovirus vehicles with deletions of other early genes such as E2, E3, E4 have been constructed to further reduce late gene expression which might be responsible for the immune response.